Lipid Analysis
Thin layer chromatography (TLC)
Chromatography is the process of separating a mixture by passing it into a solution where its components
move at different rates.
Chromatography is a technique that can be used for a wide
range of chemistry and biological processes, but for now I will tell you about
thin layer chromatography in terms of membrane proteins and separating its
lipid components that the membrane contains.
1-Sample is spotted this is called the origin and left to
dry on a glass or metal plated this is covered in a layer of silicic acid.
2-Sample components are carried up the plate by the solvent
and due to the capillary action.
3-Lipid components separate due to polarity differences.
4-more polar substances is down the plate whilst less polar
substances are up the plate.
In the next few blogs we will be discussing more about membrane proteins.
**REMEMBER TO STAY POSITIVE LIKE A PROTON!**
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