Microscopes

Types of microscopes:

Light Microscope-  Is a microscope which uses visible light to magnify and illuminate specimens which cannot be seen with visible light.

Transmission Electron Microscope-  Is a type of electron microscope which only uses electrons to magnify a specimen. electrons can go through the specimen.

Scanning Electron Microscope- Is a type of electron microscope which uses electorns to magnify a image of the surface of the specimen only.

Confocal laser scanning microscope- Is a type of microscope which uses a laser light to scan a specimen and the light is absorbed by chemicals and radiated back from the specimen and a laser is lined up in the same direction and can be focused on a small spot.

	Light	Electron microscope
		Transmission (TEM)	Scanning (SEM)
Extra info	Uses visible light to magnify specimens.	Electron beams pass through the specimen	Electron are reflected back from the specimen and are detected.
Magnification	X2000	X500,000	X100,000
Resolution (NM)	200	0.5	10
Benefits	-Inexpensive -short preparation time -can see real life colors -view living specimens	-high magnification -see intracellular details	-High magnification -3D images produced
Drawbacks	-Limited magnification -limited resolution	-very expensive -Hard to use -Images are in black  and white -Hard to prepare specimens	-very expensive -Hard to use -Images are in black  and white -Hard to prepare specimens

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