Gel Filtration Mathematics

Gel Filtration Mathematics  

Kav is easy to get and its more useful in practice (MW determination of unknown protein)

Kav= Ve-Vo/Vt-Vo

For very large molecules eluding at Ve=Vo

For very small molecules eluting at Ve=Vt

Isoelectric Point (pL)- The pH which the overall protein charged exists largely in neutral form and is influenced by a side-chain. 

A Positively charged protein pH<pL

A Negative charged protein pH>pL 

Always after protein purification stage, we need to check the protein purity and also estimate its expression yield. The nature of the amino acids in a proteins structure contribute to its surface charge. 

**REMEMBER TO STAY POSITIVE LIKE A PROTON!!**